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cynthia wolberger  (Addgene inc)


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    Addgene inc cynthia wolberger
    Cynthia Wolberger, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cynthia+wolberger/Ube1%2FPET21d+(Plasmid+%2334965)/pm39402328-288-16-18
    Average 93 stars, based on 44 article reviews
    cynthia wolberger - by Bioz Stars, 2026-10
    93/100 stars

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    Plasmid Preparation:

    Article Title: Discovery of DTX3L inhibitors through a homogeneous FRET-based assay that monitors formation and removal of poly-ubiquitin chains.
    Article Snippet: Ube1/PET21d was a gift from Cynthia Wolberger (Addgene plasmid # 34965; http://n2t.net/addgene:34965; RRID:Addgene_34965) [81]. .. UbcH5A was a gift from Cynthia Wolberger (Addgene plasmid # 61081; http://n2t.net/addgene:61081; RRID:Addgene_61081) [82]. .. Ubiquitin WT was a gift from Rachel Klevit (Addgene plasmid # 12647; http://n2 t.net/addgene:12647; RRID:Addgene_12647) [83].

    Article Title: Discovery of DTX3L inhibitors through a homogeneous FRET-based assay that monitors formation and removal of poly-ubiquitin chains.
    Article Snippet: .. Ube1/PET21d was a gift from Cynthia Wolberger (Addgene plasmid # 34965; http://n2t.net/addgene:34965; RRID:Addgene_34965) [81]. .. UbcH5A was a gift from Cynthia Wolberger (Addgene plasmid # 61081; http://n2t.net/addgene:61081; RRID:Addgene_61081) [82].

    Article Title: The Nedd4L ubiquitin ligase is activated by FCHO2-generated membrane curvature.
    Article Snippet: .. Human UbcH7 cDNA was obtained by RT-PCR and cloned in pGEX-6P. pET21d-Ube1 was a gift from Cynthia Wolberger (Addgene plasmid # 34965; http://n2t.net/addgene:34965; RRID:Addgene_34965) (Berndsen and Wolberger, 2011). pRSET-mSA was a gift from Sheldon Park (Addgene plasmid # 39860; http://n2t.net/ addgene:39860; RRID:Addgene_39860) (Lim et al, 2013). mSAαENaC constructed in pRSET-mSA to express a fusion protein of the N-terminal (aa 7–40) and C-terminal (aa 625–659) intracellular regions of αENaC (see Fig. 9A). .. Mutagenesis was performed by PCR using mutated primers and a site-directed mutagenesis kit (Stratagene).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The Nedd4L ubiquitin ligase is activated by FCHO2-generated membrane curvature.
    Article Snippet: .. Human UbcH7 cDNA was obtained by RT-PCR and cloned in pGEX-6P. pET21d-Ube1 was a gift from Cynthia Wolberger (Addgene plasmid # 34965; http://n2t.net/addgene:34965; RRID:Addgene_34965) (Berndsen and Wolberger, 2011). pRSET-mSA was a gift from Sheldon Park (Addgene plasmid # 39860; http://n2t.net/ addgene:39860; RRID:Addgene_39860) (Lim et al, 2013). mSAαENaC constructed in pRSET-mSA to express a fusion protein of the N-terminal (aa 7–40) and C-terminal (aa 625–659) intracellular regions of αENaC (see Fig. 9A). .. Mutagenesis was performed by PCR using mutated primers and a site-directed mutagenesis kit (Stratagene).

    Clone Assay:

    Article Title: The Nedd4L ubiquitin ligase is activated by FCHO2-generated membrane curvature.
    Article Snippet: .. Human UbcH7 cDNA was obtained by RT-PCR and cloned in pGEX-6P. pET21d-Ube1 was a gift from Cynthia Wolberger (Addgene plasmid # 34965; http://n2t.net/addgene:34965; RRID:Addgene_34965) (Berndsen and Wolberger, 2011). pRSET-mSA was a gift from Sheldon Park (Addgene plasmid # 39860; http://n2t.net/ addgene:39860; RRID:Addgene_39860) (Lim et al, 2013). mSAαENaC constructed in pRSET-mSA to express a fusion protein of the N-terminal (aa 7–40) and C-terminal (aa 625–659) intracellular regions of αENaC (see Fig. 9A). .. Mutagenesis was performed by PCR using mutated primers and a site-directed mutagenesis kit (Stratagene).

    Construct:

    Article Title: The Nedd4L ubiquitin ligase is activated by FCHO2-generated membrane curvature.
    Article Snippet: .. Human UbcH7 cDNA was obtained by RT-PCR and cloned in pGEX-6P. pET21d-Ube1 was a gift from Cynthia Wolberger (Addgene plasmid # 34965; http://n2t.net/addgene:34965; RRID:Addgene_34965) (Berndsen and Wolberger, 2011). pRSET-mSA was a gift from Sheldon Park (Addgene plasmid # 39860; http://n2t.net/ addgene:39860; RRID:Addgene_39860) (Lim et al, 2013). mSAαENaC constructed in pRSET-mSA to express a fusion protein of the N-terminal (aa 7–40) and C-terminal (aa 625–659) intracellular regions of αENaC (see Fig. 9A). .. Mutagenesis was performed by PCR using mutated primers and a site-directed mutagenesis kit (Stratagene).



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